Agroinoculation of Citrus tristeza virus Causes Systemic Infection and Symptoms in the Presumed Nonhost Nicotiana benthamiana
Citrus tristeza virus (CTV) naturally infects only some citrus species and relatives and within these it only invades phloem tissues.Failure to agroinfect citrus plants and the lack of an experimental herbaceous host hindered development of a workable genetic system.A full-genome cDNA of CTV isolate T36 was cloned in binary plasmids and was used to